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Table 1 Experimental and calculated MIDs of the wild-type and engineered C. glutamicum.

From: OpenFLUX: efficient modelling software for 13C-based metabolic flux analysis

   Wild-type Mutant
Metabolite fragment   Published data Present work Published data Present work
   Exp Calc Calc Exp Calc Calc
ALA 260 M0 0.508 0.509 0.509 0.523 0.525 0.525
  M1 0.353 0.354 0.354 0.341 0.342 0.342
  M2 0.106 0.106 0.106 0.103 0.104 0.104
VAL 288 M0 0.345 0.348 0.348 0.364 0.366 0.366
  M1 0.398 0.398 0.398 0.392 0.392 0.392
  M2 0.184 0.184 0.184 0.175 0.175 0.175
THR 404 M0 0.333 0.334 0.334 0.344 0.344 0.344
  M1 0.376 0.376 0.376 0.373 0.371 0.371
  M2 0.196 0.196 0.196 0.191 0.192 0.192
ASP 418 M0 0.334 0.333 0.333 0.345 0.343 0.343
  M1 0.373 0.375 0.375 0.370 0.370 0.371
  M2 0.195 0.196 0.196 0.192 0.193 0.192
GLU 432 M0 0.247 0.25 0.249 0.257 0.264 0.264
  M1 0.365 0.366 0.366 0.365 0.365 0.365
  M2 0.241 0.239 0.240 0.236 0.232 0.232
SER 390 M0 0.450 0.449 0.448 0.462 0.463 0.463
  M1 0.358 0.358 0.358 0.349 0.349 0.349
  M2 0.143 0.143 0.144 0.140 0.140 0.140
PHE 336 M0 0.271 0.274 0.274 0.287 0.289 0.289
  M1 0.382 0.381 0.381 0.380 0.381 0.381
  M2 0.228 0.228 0.228 0.220 0.220 0.220
GLY 246 M0 0.741 0.742 0.742 0.741 0.743 0.743
  M1 0.184 0.185 0.185 0.183 0.184 0.184
TYR 466 M0 0.234 0.236 0.236 0.246 0.249 0.249
  M1 0.353 0.356 0.356 0.351 0.358 0.357
  M2 0.242 0.245 0.245 0.234 0.238 0.238
TRE 361 M0 0.061 0.062 0.062 0.088 0.088 0.088
  M1 0.604 0.607 0.606 0.573 0.577 0.574
  M2 0.207 0.207 0.207 0.213 0.213 0.213
Sum weighted residues*    761 684   1735 1461
  1. * For comparative reasons, residuals resulting from minuscule technical replication errors used in original work, were accepted here.
  2. Table contains experimental and calculated MIDs published in Becker et al. [39]. The MIDs generated using OpenFLUX are consistent with the original data. The MIDs are uncorrected and have been normalized to the sum of the first n+1 peaks (n = number of backbone carbon).