Microbial Cell Factories

Table 1 Experimental and calculated MIDs of the wild-type and engineered C. glutamicum.

From: OpenFLUX: efficient modelling software for ¹³C-based metabolic flux analysis

		Wild-type			Mutant
Metabolite fragment		Published data		Present work	Published data		Present work
		Exp	Calc	Calc	Exp	Calc	Calc
ALA 260	M₀	0.508	0.509	0.509	0.523	0.525	0.525
	M₁	0.353	0.354	0.354	0.341	0.342	0.342
	M₂	0.106	0.106	0.106	0.103	0.104	0.104
VAL 288	M₀	0.345	0.348	0.348	0.364	0.366	0.366
	M₁	0.398	0.398	0.398	0.392	0.392	0.392
	M₂	0.184	0.184	0.184	0.175	0.175	0.175
THR 404	M₀	0.333	0.334	0.334	0.344	0.344	0.344
	M₁	0.376	0.376	0.376	0.373	0.371	0.371
	M₂	0.196	0.196	0.196	0.191	0.192	0.192
ASP 418	M₀	0.334	0.333	0.333	0.345	0.343	0.343
	M₁	0.373	0.375	0.375	0.370	0.370	0.371
	M₂	0.195	0.196	0.196	0.192	0.193	0.192
GLU 432	M₀	0.247	0.25	0.249	0.257	0.264	0.264
	M₁	0.365	0.366	0.366	0.365	0.365	0.365
	M₂	0.241	0.239	0.240	0.236	0.232	0.232
SER 390	M₀	0.450	0.449	0.448	0.462	0.463	0.463
	M₁	0.358	0.358	0.358	0.349	0.349	0.349
	M₂	0.143	0.143	0.144	0.140	0.140	0.140
PHE 336	M₀	0.271	0.274	0.274	0.287	0.289	0.289
	M₁	0.382	0.381	0.381	0.380	0.381	0.381
	M₂	0.228	0.228	0.228	0.220	0.220	0.220
GLY 246	M₀	0.741	0.742	0.742	0.741	0.743	0.743
	M₁	0.184	0.185	0.185	0.183	0.184	0.184
TYR 466	M₀	0.234	0.236	0.236	0.246	0.249	0.249
	M₁	0.353	0.356	0.356	0.351	0.358	0.357
	M₂	0.242	0.245	0.245	0.234	0.238	0.238
TRE 361	M₀	0.061	0.062	0.062	0.088	0.088	0.088
	M₁	0.604	0.607	0.606	0.573	0.577	0.574
	M₂	0.207	0.207	0.207	0.213	0.213	0.213
Sum weighted residues*			761	684		1735	1461

* For comparative reasons, residuals resulting from minuscule technical replication errors used in original work, were accepted here.
Table contains experimental and calculated MIDs published in Becker et al. [39]. The MIDs generated using OpenFLUX are consistent with the original data. The MIDs are uncorrected and have been normalized to the sum of the first n+1 peaks (n = number of backbone carbon).

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