Figure 5
From: Process development in Hansenula polymorpha and Arxula adeninivorans, a re-assessment
CoMed™ vector system. The basic vectors are derived from standard vectors with an engineered multiple cloning site (MCS) for the uptake of various modules. In its basic form all modules are functional in all yeasts tested so far. Module 1: ARS/CEN sequences from various sources (optional); Module 2: rDNA targeting sequences (NTS2-ETS-18SrDNA-ITS1 from various sources); Module 3: selection markers (i.e. kanMX, hph, leu2, ura3 and combinations thereof); Module 4: expression cassettes consisting of a TEF1 promoter from various sources – cloning site – terminator. The wide-range TEF1 promoter can easily be replaced by alternative strong species-specific promoters like MOX or FMD. For further details see text.