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Table 2 Effect of organic solvents on Rand protease stability

From: Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain Rand

Organic solvents Log P * Protease activity (U/ml)
   0 min 30 min
pyridine 0.71 22.66 0.0
butanol 0.80 23 38.3
benzene 2.0 61.9 72.7
toluene 2.5 45.7 47.9
p-xylene 3.1 54.4 49.6
n-hexane 3.5 47.22 53.9
n-decane 5.6 53.4 77.4
n-dodacane 6.0 65 55.7
n-tetradecane 7.6 44.7 56.4
n-hexadecane 8.8 61.9 56
None   37 37
  1. * Adopted from Laane et al. [22]
  2. Note: 25% (v/v) of organic solvents were added to the cell-free supernatant and incubated at 50°C with shaking 150 rpm for 30 min.