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Table 2 Effect of organic solvents on Rand protease stability

From: Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain Rand

Organic solvents

Log P *

Protease activity (U/ml)

  

0 min

30 min

pyridine

0.71

22.66

0.0

butanol

0.80

23

38.3

benzene

2.0

61.9

72.7

toluene

2.5

45.7

47.9

p-xylene

3.1

54.4

49.6

n-hexane

3.5

47.22

53.9

n-decane

5.6

53.4

77.4

n-dodacane

6.0

65

55.7

n-tetradecane

7.6

44.7

56.4

n-hexadecane

8.8

61.9

56

None

 

37

37

  1. * Adopted from Laane et al. [22]
  2. Note: 25% (v/v) of organic solvents were added to the cell-free supernatant and incubated at 50°C with shaking 150 rpm for 30 min.
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Microbial Cell Factories

ISSN: 1475-2859

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