Figure 5From: Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain RandEffect of pH on protease production. Bacterial cultures were adjusted to pH 4, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0, 12.0, 13.0 and incubated at 37°C with 150 rpm for 24 h. Protease activity at pH 7 was considered as 100%.Back to article page