Figure 3
From: Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production
Relative transcriptional levels of genes related to central metabolism for L-threonine producing strain MG-105 and MDS-205. Metabolic networks showing the relative transcriptional levels of genes related to the central metalbolism, mixed acid fermentation and L-threonine biosysnthetic pathways. The numbers are the relative ratio of the expression level of MDS-205 as compared to that of MG-105. Metabolite abbreviations: GLCout, glucose in medium; GLCcyt, glucose in cytoplasm; G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; FBP, fructose-1,6-bisphosphate; DHAP, dihydroxyacetonephosphate; GAP, glyceraldehyde-3-phosphate; GBP, 1,3-bisphosphoglycerate; 3PGA, 3-phosphoglycerate; 2PGA, 2-phosphoglycerate; PEP, phosphoenolpyruvate; PYR, pyruvate; AcCoA, acetyl-CoA; ACP, acetyl-phosphate; ACE, acetate; ETH, ethanol; OAA, oxaloacetate; CIT, citrate; ICIT, isocitrate; AKG, a-ketoglutarate; SUCOA, succinyl-CoA; SUC, succinate; FUM, fumarate; MAL, malate; ASP, aspartate; A4P, aspartyl-4-phosphate; ASA, aspartate semialdehyde; HS, homoserine; PHS, homoserine phosphate; THRout, L-threonine in medium; THRcyt, L-threonine in cytoplasm; 6PGL, gluconolactone-6-phosphate; 6PGC, 6-phosphogluconate; RU5P, ribulose-5-phosphate; X5P, xylurose-5-phosphate; R5P, ribose-5-phosphate; S7P, sedoheptulose-7-phosphate; E4P, erythrose-4-phosphate.