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Table 3 Escherichia coli strains and plasmids used in this work

From: Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli

Strain/Plasmid Characteristics Reference
Strains   
PB11 JM101 [supE, thi, Δ(lac-proAB), F'] ΔptsHI, crr::kmR, glucose- [10]
W3110 trpD9923 W3110 [F-λ- INV (rrn D-rrn E) 1] tryptophan auxotroph, randomly mutagenized by treatment with ultraviolet radiation. [5]
W3110 trpD9923 PTS- As W3110 trpD9923 but ΔptsHI, crr::kmR, glucose- This work
Plasmids   
pJLBaroGfbr aroGfbr expressed from the lacUV5 promoter, lacIq and tet genes, tetracycline resistance, pACYC184 replication origin. [14]
pv5Glk5GalP glk and galP genes expressed from the trc5 promoter, spectinomycin resistance. pCL1920 replication origin. [13]
pJLBaroGfbrtkt A pJLBaroGfbr derivative, containing the tktA gene with its native promoter. This work