Table 3 Escherichia coli strains and plasmids used in this work

JM101 [supE, thi, Δ(lac-proAB), F'] ΔptsHI, crr::km^R, glucose^-

[10]

W3110 [F^-λ^- INV (rrn D-rrn E) 1] tryptophan auxotroph, randomly mutagenized by treatment with ultraviolet radiation.

[5]

As W3110 trpD9923 but ΔptsHI, crr::km^R, glucose^-

This work

aroG^fbr expressed from the lacUV5 promoter, lacI^q and tet genes, tetracycline resistance, pACYC184 replication origin.

[14]

glk and galP genes expressed from the trc5 promoter, spectinomycin resistance. pCL1920 replication origin.

[13]

pJLBaroG^fbr derivative, containing the tktA gene with its native promoter.

This work