Skip to main content

Advertisement

Table 2 Comparison of kinetic and fermentation parameters of E. coli W3110trpD9923 derivative strains in fed-batch fermentor cultures

From: Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli

Strain Final biomass
(gDCW/L)
μ
(h-1)
q Glc
(gGlc/gDCW·h)
q Ant
(gAnt/gDCW·h)
YBiom/Glc
(gDCW/gGlc)
YAnt/Glc
(gAnt/gGlc)
Final anthranilate titer
(g/L)
Final acetate titer
(g/L)
W3110 trpD9923 21.35 ± 3.76
[34 h]a
0.25 ± 0.02
[2–10 h]b
0.17 ± 0.01
[0–34 h]
0.01 ± 0.00
[6–30 h]
0.27 ± 0.02
[0–34 h]
0.08 ± 0.01
[6–30 h]
4.21 ± 0.03 9.65 ± 2.17
W3110 trpD9923 PTS - 23.30 ± 0.56
[62 h]
0.10 ± 0.00
[6–18 h]
0.06 ± 0.00
[0–62 h]
0.01 ± 0.00
[14–72 h]
0.28 ± 0.00
[0–62 h]
0.15 ± 0.00
[14–72 h]
13.30 ± 0.89 NDc
W3110 trpD9923 PTS - /pv5Glk5GalP 14.66 ± 1.26
[38 h]
0.15 ± 0.00
[6–18 h]
0.13 ± 0.02
[0–38 h]
0.01 ± 0.00
[10–34 h]
0.20 ± 0.03
[0–38 h]
0.09 ± 0.00
[10–34 h]
5.30 ± 0.07 ND
W3110 trpD9923 /pJLB aroG fbr 19.47 ± 0.12
[34 h]
0.21 ± 0.00
[2–14 h]
0.12 ± 0.01
[0–30 h]
0.01 ± 0.00
[2–38 h]
0.28 ± 0.02
[0–30 h]
0.08 ± 0.002
[2–38 h]
6.72 ± 0.01 3.18 ± 0.52
W3110 trpD9923 /pJLB aroG fbr tkt A 18.54 ± 0.19
[34 h]
0.18 ± 0.01
[6–18 h]
0.14 ± 0.01
[0–34 h]
0.02 ± 0.00
[2–34 h]
0.24 ± 0.02
[0–34 h]
0.20 ± 0.00
[2–34 h]
14.00 ± 0.07 0.50 ± 0.1
W3110 trpD9923 PTS - /pJLB aroG fbr tkt A 11.12 ± 0.25
[38 h]
0.13 ± 0.00
[10–26 h]
0.16 ± 0.01
[0–34 h]
0.02 ± 0.00
[6–38 h]
0.19 ± 0.02
[0–34 h]
0.11 ± 0.00
[6–38 h]
7.16 ± 0.53 ND
W3110 trpD9923 PTS - /pv5Glk5GalP/pJLB aroG fbr tkt A 15.40 ± 0.14
[38 h]
0.16 ± 0.00
[10–22 h]
0.15 ± 0.01
[0–30 h]
0.02 ± 0.01
[10–34 h]
0.23 ± 0.02
[0–30 h]
0.09 ± 0.01
[10–34 h]
5.08 ± 0.06 ND
  1. a values in brackets indicate the time maximum biomass was achieved b values in brackets indicate the period considered for calculation of kinetic parameters. c ND, non detectable levels.