Table 2 Comparison of kinetic and fermentation parameters of E. coli W3110trpD9923 derivative strains in fed-batch fermentor cultures
From: Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli
Strain | Final biomass (gDCW/L) | μ (h-1) | q Glc (gGlc/gDCW·h) | q Ant (gAnt/gDCW·h) | YBiom/Glc (gDCW/gGlc) | YAnt/Glc (gAnt/gGlc) | Final anthranilate titer (g/L) | Final acetate titer (g/L) |
|---|---|---|---|---|---|---|---|---|
W3110 trpD9923 | 21.35 ± 3.76 [34 h]a | 0.25 ± 0.02 [2–10 h]b | 0.17 ± 0.01 [0–34 h] | 0.01 ± 0.00 [6–30 h] | 0.27 ± 0.02 [0–34 h] | 0.08 ± 0.01 [6–30 h] | 4.21 ± 0.03 | 9.65 ± 2.17 |
W3110 trpD9923 PTS - | 23.30 ± 0.56 [62 h] | 0.10 ± 0.00 [6–18 h] | 0.06 ± 0.00 [0–62 h] | 0.01 ± 0.00 [14–72 h] | 0.28 ± 0.00 [0–62 h] | 0.15 ± 0.00 [14–72 h] | 13.30 ± 0.89 | NDc |
W3110 trpD9923 PTS - /pv5Glk5GalP | 14.66 ± 1.26 [38 h] | 0.15 ± 0.00 [6–18 h] | 0.13 ± 0.02 [0–38 h] | 0.01 ± 0.00 [10–34 h] | 0.20 ± 0.03 [0–38 h] | 0.09 ± 0.00 [10–34 h] | 5.30 ± 0.07 | ND |
W3110 trpD9923 /pJLB aroG fbr | 19.47 ± 0.12 [34 h] | 0.21 ± 0.00 [2–14 h] | 0.12 ± 0.01 [0–30 h] | 0.01 ± 0.00 [2–38 h] | 0.28 ± 0.02 [0–30 h] | 0.08 ± 0.002 [2–38 h] | 6.72 ± 0.01 | 3.18 ± 0.52 |
W3110 trpD9923 /pJLB aroG fbr tkt A | 18.54 ± 0.19 [34 h] | 0.18 ± 0.01 [6–18 h] | 0.14 ± 0.01 [0–34 h] | 0.02 ± 0.00 [2–34 h] | 0.24 ± 0.02 [0–34 h] | 0.20 ± 0.00 [2–34 h] | 14.00 ± 0.07 | 0.50 ± 0.1 |
W3110 trpD9923 PTS - /pJLB aroG fbr tkt A | 11.12 ± 0.25 [38 h] | 0.13 ± 0.00 [10–26 h] | 0.16 ± 0.01 [0–34 h] | 0.02 ± 0.00 [6–38 h] | 0.19 ± 0.02 [0–34 h] | 0.11 ± 0.00 [6–38 h] | 7.16 ± 0.53 | ND |
W3110 trpD9923 PTS - /pv5Glk5GalP/pJLB aroG fbr tkt A | 15.40 ± 0.14 [38 h] | 0.16 ± 0.00 [10–22 h] | 0.15 ± 0.01 [0–30 h] | 0.02 ± 0.01 [10–34 h] | 0.23 ± 0.02 [0–30 h] | 0.09 ± 0.01 [10–34 h] | 5.08 ± 0.06 | ND |