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Table 1 Comparison of kinetic and fermentation parameters of E. coli W3110trpD9923 derivative strains in flask cultures

From: Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli

Strain Final biomass
(gDCW/L)
μ
(h-1)
q Glc
(gGlc/gDCW·h)
q Ant
(gAnt/gDCW·h)
YBiom/Glc
(gDCW/gGlc)
YAnt/Glc
(gAnt/gGlc)
Final anthranilate titer (g/L)
W3110 trpD9923 1.29 ± 0.03
[16 h]a
0.26 ± 0.04
[0–12 h]b
0.34 ± 0.01
[0–16 h]
0.02 ± 0.00
[8–20 h]
0.18 ± 0.01
[0–16 h]
0.06 ± 0.01
[8–20 h]
0.31 ± 0.01
W3110 trpD9923 PTS - 1.04 ± 0.05
[44 h]
0.09 ± 0.01
[24–36 h]
0.13 ± 0.01
[0–44 h]
0.03 ± 0.00
[28–52 h]
0.18 ± 0.01
[0–44 h]
0.12 ± 0.01
[28–52 h]
0.70 ± 0.07
W3110 trpD9923 PTS - /pv5Glk5GalP 0.94 ± 0.05
[32 h]
0.20 ± 0.01
[8–16 h]
0.24 ± 0.03
[0–20 h]
0.04 ± 0.00
[8–16 h]
0.15 ± 0.02
[0–20 h]
0.12 ± 0.00
[8–16 h]
0.33 ± 0.01
W3110 trpD9923 /pJLB aroG fbr 1.09 ± 0.01
[16 h]
0.20 ± 0.00
[0–12 h]
0.30 ± 0.01
[0–12 h]
0.07 ± 0.00
[4–12 h]
0.28 ± 0.01
[0–12 h]
0.16 ± 0.01
[4–12 h]
0.44 ± 0.00
W3110 trpD9923 /pJLB aroG fbr tkt A 0.93 ± 0.04
[20 h]
0.24 ± 0.00
[0–12 h]
0.37 ± 0.02
[0–16 h]
0.07 ± 0.00
[8–20 h]
0.17 ± 0.01
[0–16 h]
0.20 ± 0.05
[8–20 h]
0.75 ± 0.04
W3110 trpD9923 PTS - /pJLB aroG fbr tkt A 0.39 ± 0.00
[32 h]
0.15 ± 0.01
[4–16 h]
0.43 ± 0.04
[0–24 h]
0.03 ± 0.00
[12–44 h]
0.10 ± 0.01
[0–24 h]
0.10 ± 0.02
[12–44 h]
0.45 ± 0.02
W3110 trpD9923 PTS - /pv5Glk5GalP/pJLB aroG fbr tkt A 0.79 ± 0.03
[24 h]
0.24 ± 0.00
[4–12 h]
0.21 ± 0.00
[0–28 h]
0.020 ± 0.00
[20–40 h]
0.19 ± 0.00
[0–28 h]
0.14 ± 0.03
[20–40 h]
0.33 ± 0.02
  1. a values in brackets indicate the time maximum biomass was achieved b values in brackets indicate the period considered for calculation of kinetic parameters.