Table 1 Comparison of kinetic and fermentation parameters of E. coli W3110trpD9923 derivative strains in flask cultures
From: Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli
Strain | Final biomass (gDCW/L) | μ (h-1) | q Glc (gGlc/gDCW·h) | q Ant (gAnt/gDCW·h) | YBiom/Glc (gDCW/gGlc) | YAnt/Glc (gAnt/gGlc) | Final anthranilate titer (g/L) |
|---|---|---|---|---|---|---|---|
W3110 trpD9923 | 1.29 ± 0.03 [16 h]a | 0.26 ± 0.04 [0–12 h]b | 0.34 ± 0.01 [0–16 h] | 0.02 ± 0.00 [8–20 h] | 0.18 ± 0.01 [0–16 h] | 0.06 ± 0.01 [8–20 h] | 0.31 ± 0.01 |
W3110 trpD9923 PTS - | 1.04 ± 0.05 [44 h] | 0.09 ± 0.01 [24–36 h] | 0.13 ± 0.01 [0–44 h] | 0.03 ± 0.00 [28–52 h] | 0.18 ± 0.01 [0–44 h] | 0.12 ± 0.01 [28–52 h] | 0.70 ± 0.07 |
W3110 trpD9923 PTS - /pv5Glk5GalP | 0.94 ± 0.05 [32 h] | 0.20 ± 0.01 [8–16 h] | 0.24 ± 0.03 [0–20 h] | 0.04 ± 0.00 [8–16 h] | 0.15 ± 0.02 [0–20 h] | 0.12 ± 0.00 [8–16 h] | 0.33 ± 0.01 |
W3110 trpD9923 /pJLB aroG fbr | 1.09 ± 0.01 [16 h] | 0.20 ± 0.00 [0–12 h] | 0.30 ± 0.01 [0–12 h] | 0.07 ± 0.00 [4–12 h] | 0.28 ± 0.01 [0–12 h] | 0.16 ± 0.01 [4–12 h] | 0.44 ± 0.00 |
W3110 trpD9923 /pJLB aroG fbr tkt A | 0.93 ± 0.04 [20 h] | 0.24 ± 0.00 [0–12 h] | 0.37 ± 0.02 [0–16 h] | 0.07 ± 0.00 [8–20 h] | 0.17 ± 0.01 [0–16 h] | 0.20 ± 0.05 [8–20 h] | 0.75 ± 0.04 |
W3110 trpD9923 PTS - /pJLB aroG fbr tkt A | 0.39 ± 0.00 [32 h] | 0.15 ± 0.01 [4–16 h] | 0.43 ± 0.04 [0–24 h] | 0.03 ± 0.00 [12–44 h] | 0.10 ± 0.01 [0–24 h] | 0.10 ± 0.02 [12–44 h] | 0.45 ± 0.02 |
W3110 trpD9923 PTS - /pv5Glk5GalP/pJLB aroG fbr tkt A | 0.79 ± 0.03 [24 h] | 0.24 ± 0.00 [4–12 h] | 0.21 ± 0.00 [0–28 h] | 0.020 ± 0.00 [20–40 h] | 0.19 ± 0.00 [0–28 h] | 0.14 ± 0.03 [20–40 h] | 0.33 ± 0.02 |