Table 1 Composition of the media used in this study

Complex

Medium 707

5 g peptone, 3 g yeast extract, 1 g MgSO₄·7 H₂O

pH 7.0

Seed medium (SM)

4 g peptone, 4 g yeast extract, 2 g KH₂PO₄, 4 g K₂HPO₄, 0.5 g MgSO₄7H₂0, 10 g glucose

pH 7.2

R3/1

5 g yeast extract, 0.1 g casamino acids, 3 g L-proline, 10 g MgCl₂·6H₂O, 4 g CaCl₂·2H₂O, 0.2 g K₂SO₄, 0.05 g KH₂PO₄, 5.6 g TES, 10 g glucose

pH 7.2

Medium 266 (YS)

2 g yeast extract, 10 g soluble starch

pH 7.3

OMY

40 g oatmeal, 1 g yeast extract

pH 6.8–7.0

Nutrient broth

3 g beef extract, 5 g tryptone, 15 g NaCl

pH 7.2

Chemically defined

MM-101

7 g NH₄Cl, 3 g KH₂PO₄, 7 g K₂HPO₄, 0.25 g MgSO₄·7 H₂O, 0.0138 g CaCl₂·2 H₂O, 10 g glucose, 2 ml trace solution element (TSE)^a

pH 6.9

MM-102

1 g NH₄Cl, 1 g CaCO₃, 0.5 g NaCl, 0.4 g MgSO₄·7 H₂O, 0.15 g KH₂PO₄, 0.35 g K₂HPO₄, 10 g glucose, 2 ml trace solution element (TSE)^a

pH 6.9