Microbial Cell Factories

Table 1 HBsAg production with P. pastoris

From: Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen

Strain (copy#)	MeOH (%)	Induction time (h)	VLP (method)^a	Quantification	Yield (g L^-1)	Reference
Mut^s (1)	0.5	200	+ (RIA; EM)	Western	0.4	[11]
Mut^s (8)	0.1	192	+ (E)	Western	1.0	[10]
nd (nd)^b	nd	nd	Nd	E	0.097	[12]
Mut^s (nd)	0.5	96	+ (EM)	nd	nd	[9]
Mut^s (nd)	1.0	72	Nd	RPHA^c	0.0125	[8]
Mut^s (nd)	0.1	70	Nd	E	0.32	[13]
Mut⁺ (nd)	0.25	50	Nd	E	0.35	[13]
Mut^s (8)	0.6	90–160	+ (EM)	RP-HPLC	6–7	present study

^a The '+' symbol denotes detection of virus-like particles (VLP) using radioimmunoassay (RIA), electron microscopy (EM) or ELISA (E)
^b nd indicates no data available
^c RPHA: reversed passive hemeagglutination assay

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ISSN: 1475-2859

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