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Table 1 HBsAg production with P. pastoris

From: Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen

Strain (copy#) MeOH (%) Induction time (h) VLP (method)a Quantification Yield (g L-1) Reference
Muts (1) 0.5 200 + (RIA; EM) Western 0.4 [11]
Muts (8) 0.1 192 + (E) Western 1.0 [10]
nd (nd)b nd nd Nd E 0.097 [12]
Muts (nd) 0.5 96 + (EM) nd nd [9]
Muts (nd) 1.0 72 Nd RPHAc 0.0125 [8]
Muts (nd) 0.1 70 Nd E 0.32 [13]
Mut+ (nd) 0.25 50 Nd E 0.35 [13]
Muts (8) 0.6 90–160 + (EM) RP-HPLC 6–7 present study
  1. a The '+' symbol denotes detection of virus-like particles (VLP) using radioimmunoassay (RIA), electron microscopy (EM) or ELISA (E)
  2. b nd indicates no data available
  3. c RPHA: reversed passive hemeagglutination assay