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Figure 2 | Microbial Cell Factories

Figure 2

From: Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen

Figure 2

Time-course analysis of HBsAg production. (A) Total cell lysates analyzed by SDS-PAGE from samples taken directly before the addition of methanol (time 0) and 2, 4, 6, 8, 10, 22, 30, 44, 104, and 164 hours after the onset of methanol feeding. (B) Western blot analysis of total cell lysates taken directly before the addition of methanol (time 0) and 22, 44, 104, and 164 hours after the onset of methanol feeding using a commercially available rabbit anti-HBsAg antiserum. The bidirectional arrow between panels A and B denotes the position of the HBsAg. (C) Transmission electron microscopic survey of negatively stained, purified HBsAg particles.

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