Loading plots of PLS-DA analysis of (A) reference and pat1Δ, (B) reference and lsm1Δ and (C) reference and dhh1Δ (each one n=6) cell extracts from GC/MS analysis using normalized peak areas. Error-bars indicate jack-knifed confidence intervals. Values below zero indicate a higher concentration compared to the reference strain. Values above zero denote lower concentrations compared to the reference strain. Data were pareto scaled and analysis was carried out using SIMCA-P+ 11.5 (Umetrics, Malmö, Sweden).