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Figure 3 | Microbial Cell Factories

Figure 3

From: Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD)

Figure 3

Lipase activity of transformants harboring tliA-fusion gene. Semi-quantitative estimation of the extracellular secretion of target protein containing lipase (TliA) was performed by comparing the sizes of lipolytic clear halos. E. coli transformants harboring tliA-fusion gene were prepared in two sets; with P. fluorescens ABC transporter (pABC-ACYC) and without ABC transporter (pACYC-184). After E. coli cells were incubated on the LAT plate at 25°C for 48 h, halos were observed; 1, E. coli (pTliA); 2, E. coli (pGFP-TliA); 3, E. coli (pEGF-TliA); 4, E. coli (pCTP-TliA); 5, E. coli (pTliA-CTP); 6, E. coli (pCTP-GFP-TliA).

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