Figure 2

Green fluorescence of GFP-fusion proteins. A. Green fluorescence of GFP-fusion proteins without ABC transporter. E. coli harboring pGFPuv, pGFP-TliA, pGFP-LARD0, pGFP-LARD1 and pGFP-SXold (intermediate plasmid making the fusion proteins of GFP and a few additional amino acids) was streaked on LB containing ampicillin and grown at 37°C or 25°C. B. Green fluorescence under fluorescence microscope. E. coli harboring pGFP-LARD0 was viewed with a fluorescence microscope and photographs were obtained under light and excitation filter conditions with 1,000 magnification. C. Green fluorescence of GFP-fusion proteins with ABC transporters. E. coli harboring the GFP-fusion plasmids pGFP-TliA (TliA), pGFP-LARD0 (LARD0), or pGFP-LARD1 (LARD1) was viewed under UV illumination with or without ABC transporters. pACYC-184, pABC-ACYC, and pEcPrtDEF-184 lacked transporter genes, or had the genes of TliDEF or EcPrtDEF as the ABC transporter, respectively.