Figure 5
From: Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield
Polysome profiles for the yTHC BMS1 and wild-type strains. (A) Polysome OD254 profiles for yeast strains producing Fps1 in the absence (upper panels) or presence (lower panels) of 0.5 μg/mL doxycycline. Polysome peaks containing 2, 3 and 4 ribosomes are visible. (B) Ribosome disassociation profiles at OD254 for 50 mM EDTA-treated samples of wild-type and yTHCBMS1 strains producing Fps1 in the absence and presence of 0.5 μg/mL doxycycline (upper and lower panels respectively). Changes in peak heights and ratios of 60S and 40S subunits for all four conditions are tabulated.