Figure 4
From: Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield
Calorimetric profiles for the yTHC BMS1 and wild-type strains. Profiles are shown for yTHCBMS1 producing Fps1 in the absence (white circles) or presence (black circles) of 0.5 μg/mL doxycycline. Those for the wild-type in the absence (white squares) or presence (black squares) of 0.5 μg/mL doxycycline overlay the former trace. The top inset panel shows the glucose (squares) and ethanol (circles) data for the yTHCBMS1 strain producing Fps1 in the absence (white symbols) or presence (black symbols) of 0.5 μg/mL doxycycline. The lower inset shows the natural log plot of the glucose phase data for the yTHCBMS1 strain, showing the linear fit used to calculate rate constants of 0.32 h-1 and 0.21 h-1 in the absence (white circles) or presence (black circles) of 0.5 μg/mL doxycycline, respectively.