Skip to main content

Table 2 Integration sites of myxochromide biosynthetic genes in C. macrosporus GT-2.

From: Novel expression hosts for complex secondary metabolite megasynthetases: Production of myxochromide in the thermopilic isolate Corallococcus macrosporus GT-2

  DNA sequence   
Mutant clone IR (underlined) insertion site (bold italic) Gene product Organism, Identity, %
GT2::mch ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA
GTTGGAGAGCGAGACGACGACGAGCT---
NADP
oxidoreductase
Myxococcus xanthus DK1622, 91
P1 ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA
CACCTGTGCAGCCGGGCTCACCCCGG---
Hypothetical protein Myxococcus xanthus DK1622, 79
P2 ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA
GATGGCGCTGACGCGAATGATGACCT---
site-specific recombinase, phage integrase family protein Mycobacterium avium 104, 31
P3 ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA
GCCCAGGCCCGCTTCACCGCCAATGG---
Predicted transcriptional regulator Magnetospirillum magneticum
AMB-1, 60
P5 ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA
GTCATGGACCTCCGTGACGGTGCCCA---
excinuclease ABC,
A subunit
Myxococcus xanthus DK1622, 98
P6 ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA
CGTGCCCATCGACGCCTCACCGGCGA---
hydrolase, CocE/NonD family Myxococcus xanthus DK1622, 72