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Table 2 Integration sites of myxochromide biosynthetic genes in C. macrosporus GT-2.

From: Novel expression hosts for complex secondary metabolite megasynthetases: Production of myxochromide in the thermopilic isolate Corallococcus macrosporus GT-2

  DNA sequence   
Mutant clone IR (underlined) insertion site (bold italic) Gene product Organism, Identity, %
GT2::mch ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA GTTGGAGAGCGAGACGACGACGAGCT--- NADP oxidoreductase Myxococcus xanthus DK1622, 91
P1 ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA CACCTGTGCAGCCGGGCTCACCCCGG--- Hypothetical protein Myxococcus xanthus DK1622, 79
P2 ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA GATGGCGCTGACGCGAATGATGACCT--- site-specific recombinase, phage integrase family protein Mycobacterium avium 104, 31
P3 ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA GCCCAGGCCCGCTTCACCGCCAATGG--- Predicted transcriptional regulator Magnetospirillum magneticum AMB-1, 60
P5 ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA GTCATGGACCTCCGTGACGGTGCCCA--- excinuclease ABC, A subunit Myxococcus xanthus DK1622, 98
P6 ---AGCGTGAAGACCGGGGACTTATCAGCCAACCTGT TA CGTGCCCATCGACGCCTCACCGGCGA--- hydrolase, CocE/NonD family Myxococcus xanthus DK1622, 72