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Table 3 Comparison of xylose fermentation by the recombinant S. cerevisiae strains BP000 and BP10001

From: Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae

  BP000 BP10001
  Shake flask (oxygen limited) Bioreactor (anaerobic) Shake flask (oxygen limited) Bioreactor (anaerobic)
q xylose a 0.07 0.06 0.07 0.08 d
Y ethanol b 0.24 0.24 0.34 0.34
Y xylitol 0.35 0.39 0.17 0.19
Y glycerol 0.091 0.048 0.063 0.021
Y acetate 0.019 0.019 0.031 0.020
C-recovery 93%c 101% 94%c 96%
  1. a Xylose uptake rates (q) are given in g/g CDW/h
  2. b Yields (Y) are given in g/g xylose.
  3. c For the calculation of the carbon balance, it was assumed that one mole of CO2 was formed per mol of ethanol or acetate
  4. The given values were obtained by analyzing samples taken in the range 45 – 60% xylose consumption. Shake flask experiments were done in triplicates, bioreactor experiments in duplicate. Mean values are shown. Their relative S.D. was < 5% with the exception of values of qxylose and Yacetate from shake flasks experiments whose S.D. was ≤ 14%. d he S.D. for this value was about 30%.