Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae

Table 3 Comparison of xylose fermentation by the recombinant S. cerevisiae strains BP000 and BP10001

^a Xylose uptake rates (q) are given in g/g CDW/h
^b Yields (Y) are given in g/g xylose.
^c For the calculation of the carbon balance, it was assumed that one mole of CO₂ was formed per mol of ethanol or acetate
The given values were obtained by analyzing samples taken in the range 45 – 60% xylose consumption. Shake flask experiments were done in triplicates, bioreactor experiments in duplicate. Mean values are shown. Their relative S.D. was < 5% with the exception of values of q_xylose and Y_acetate from shake flasks experiments whose S.D. was ≤ 14%. ^d he S.D. for this value was about 30%.

ISSN: 1475-2859