Skip to main content

Table 2 XR, XDH and XK activities in crude cell extracts of BP000 and BP10001. Cells were grown aerobically on a mixed sugar substrate containing 20 g/L of each glucose and xylose and were then disrupted with Y-Per.

From: Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae

Strain

XR activity U/mga

XDH activity U/mg

XK activity U/mg

BP000

NADH

0.15 ± 0.01b

1.1 ± 0.1

1.7 ± 0.2

 

NADPH

0.18 ± 0.01

  

BP10001

NADH

0.26 ± 0.01

1.3 ± 0.1

2.4 ± 0.1

 

NADPH

0.33 ± 0.01

  

Cen.PK 113-7D

NADH

n.d.c

n.d.

0.14 ± 0.04

 

NADPH

0.008 ± 0.003

  
  1. a Activities measured using a coenzyme concentration of 350 μM and a xylose concentration of 700 mM
  2. b Mean values ± S.D. from three independent experiments including cultivation and disruption of cells and measurement of protein and activity
  3. c n.d. not detectable.
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us