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Table 2 XR, XDH and XK activities in crude cell extracts of BP000 and BP10001. Cells were grown aerobically on a mixed sugar substrate containing 20 g/L of each glucose and xylose and were then disrupted with Y-Per.

From: Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae

Strain XR activity U/mga XDH activity U/mg XK activity U/mg
BP000 NADH 0.15 ± 0.01b 1.1 ± 0.1 1.7 ± 0.2
  NADPH 0.18 ± 0.01   
BP10001 NADH 0.26 ± 0.01 1.3 ± 0.1 2.4 ± 0.1
  NADPH 0.33 ± 0.01   
Cen.PK 113-7D NADH n.d.c n.d. 0.14 ± 0.04
  NADPH 0.008 ± 0.003   
  1. a Activities measured using a coenzyme concentration of 350 μM and a xylose concentration of 700 mM
  2. b Mean values ± S.D. from three independent experiments including cultivation and disruption of cells and measurement of protein and activity
  3. c n.d. not detectable.