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Figure 2 | Microbial Cell Factories

Figure 2

From: Metabolic responses to pyruvate kinase deletion in lysine producing Corynebacterium glutamicum

Figure 2

Experimental design for quantification of flux parameters at the pyruvate node of Corynebacterium glutamicum with an equimolar mixture of [13C 6 ] glucose and naturally labelled glucose. Relative change of the mass isotopomer distribution of aspartate (m/z 418) with varied ΦPEPC (A), relative change of the mass isotopomer distribution of valine (m/z 288) with varied ζPEPC/PEPCK (B), relative change of the mass isotopomer distribution of alanine (m/z 260) with varied ζPC/MAE (C). The labelling patterns at sole contribution of PEPC (ΦPEPC = 100 %), and highly reversible fluxes at the pyruvate node (ζPEPC/PEPCK = ζPC/MAE = 10) are taken as reference point and set to 100 %. The flux parameters investigated here comprise ΦPEPC (flux partitioning between PEPC and PC), ζPEPC/PEPCK (ratio of exchange flux to net flux between the pools of PEP and OAA/MAL catalyzed by PEPC and PEPCK) and ζPC/MAE (ratio of exchange flux to net flux between the pools of PYR and OAA/MAL catalyzed by PC and MAE). The exact definitions for the flux parameters are given in the appendix. Unless varied the flux parameters reflect the situation for the parent strain C. glutamicum lysCfbr (Figure 4).

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