Schematic description of the construct designed to express His
-tagged mature SnOLP in E. coli. The regions of the preproprotein SnOLP are shown. The positions of the primers used to generate fragments coding for mature SnOLP form are shown. The orientations of the primers PPM1 and PPM2, including the respective restriction cloning sites, are indicated by arrows. The His6-tag encoded by the pQE30 vector is indicated. SP, Signal Peptide; CT, Carboxy-Terminal propeptide; M, Methionine; *, Stop codon.