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Figure 2 | Microbial Cell Factories

Figure 2

From: Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes

Figure 2

Influence of different sample treatments on Ct values of plasmids and chromosomes in culture of Escherichia coli BL21 (DE3) containing pET3a plasmid with gene for hG-CSF from bioprocess in GYSP medium. Sample treatments are marked as N nontreated, C centrifuged, F frozen, 95 and 99 temperature treatment in °C and are applied in subsequent steps as preformed. Duration of heating for 10 min, 15 min, 20 min at 95°C and 99°C are not presented separately.

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