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Figure 1 | Microbial Cell Factories

Figure 1

From: Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes

Figure 1

Accumulation level of hG-CSF in fermentations at 25°C without ampicillin (a) and with ampicillin (b). Escherichia coli BL21 (DE3) strain transformed with pET3a plasmid with gene for hG-CSF was used. Samples were taken every four hours, bands corresponding to hG-CSF are framed. M stands for Molecular weight standard Mark 12 with (Invitrogen) with purified hG-CSF; S stands for standard, purified hG-CSF.

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