Multiple alignment of the partial Amino acid sequences containing the conserved motifs of GxSxG and putative catalytic triad resides of esterases. Except for Pye3, other protein sequences were retrieved from GenBank under following accession numbers: our metagenome (FJ389548), Marinobacter aquaeolei VT8 (ABM19331), Shewanella halifaxensis HAW-EB4 (ABZ78572), Nitrosococcus oceani ATCC 19707 (ABA57886), Shewanella loihica PV-4 (ABO23408), Rhodospirillum rubrum ATCC 11170 (ABC24200). The alignment was carried out using the Clustal W method. The open boxes indicate amino acid resides belonging to the putative catalytic triad resides, triangles denote the active site. The same amino acid resides are marked by (*).