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Table 2 Initial rates and ee-values of ethyl 4-cyanobenzoylformate reduction by different whole-cell biocatalysts.

From: Whole-cell bioreduction of aromatic α-keto esters using Candida tenuis xylose reductase and Candida boidinii formate dehydrogenase co-expressed in Escherichia coli

strain cell dry weight [g/L] substrate conc. initial rate [mmol/g h]1/ee2 [%]
E. coli XR_FDH 5 10 0.60/97.0 R
E. coli XR_FDH 5 100 1.12/99.0 R
E. coli XR_FDH 20 100 1.39/99.3 R
E. coli XR_FDH 40 100 1.19/98.6 R
E. coli XR_FDH 40 500 1.94/99.9 R
S. cerevisiae XR2μ 3 5 10 0.26/≥ 99.9 R
S. cerevisiae XR2μ 3 40 100 1.03/≥ 99.9 R
S. cerevisiae wild-type3 5 10 0.06/80.4 R
  1. 1Determined from the time course of substrate conversion between 0 and ≤ 1.4 h reaction time; standard errors ≤ 20%. 2 The detection limit of ethyl 4-cyanomandelate is below 0.01 mM. 3Anaerobic reaction conditions where [O2] < 15 μM; ethanol was used as co-substrate.