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Figure 2 | Microbial Cell Factories

Figure 2

From: Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks

Figure 2

Optimization of the novel glucose delivery system. (a) Effect of the regulating gel and its composition on the accumulation of starch in the culture medium. Starch diffusion from the storage gel was investigated in microwell plates without addition of glucoamylase and with sterile liquid medium. The control was without regulating gel. (b) Glucose accumulation from a two-phase gel into sterile liquid medium with different amounts of glucoamylase (0.15–30 AGU l-1). (c) Comparison of the glucose release rates from figure (b). For the experiments the microwell plates were incubated at 37°C at a shaking speed of 750 rpm. Whole wells were harvested for each analysis.

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