Figure 3From: Conversion of β-carotene into astaxanthin: Two separate enzymes or a bifunctional hydroxylase-ketolase protein?HPLC analysis of β-carotene and xanthophylls produced by A) X. dendrorhous ATCC 96815 or B) Mucor circinelloides MS12, before and after transformation with the crtS gene. CR, cryptoxanthin; ZX, zeaxanthin; AX, astaxanthin; HDCO, 3-hydroxy-3',4'-didehydro-β-ψ-carotene-4-one. Note that expression of the crtS gene in M. circinelloides results in the production of β-carotene hydroxylated derivatives only, whereas transformants of X. dendrorhous with the same gene produce astaxanthin in addition to the hydroxylated derivatives.Back to article page