Table 1 Oligonucleotide primers and probes used in this study.
From: Sample processing for DNA chip array-based analysis of enterohemorrhagic Escherichia coli (EHEC)
Name | Function (Accession number) | 5' position | Sequence (5' – 3')a |
|---|---|---|---|
gene etpC (AF401292) | |||
O157 Primer I | etpC upper primer | 35621 → | ATTATGTTGTTCTTTCTATCATTCC |
O157 Primer II | etpC lower primer | 35815 ← | TTGATCACCAGTTACCGCTGTTTCC |
O157 cp | etpC capture probe | 35723 → | X - (ACCT)CTTATTGCCGGATATCAGCTGGTGT |
O157 dp | etpC detection probe | 35749 → | GGTTATCCATCATTTCTGGCTGACT(GTCA) - Y |
gene stx1 (M17358) | |||
Stx1 Primer I | stx1 upper primer | 272 → | CGCTGAATGTCATTCGCTCTGC |
Stx1 Primer II | stx1 lower primer | 553 ← | CGTGGTATAGCTACTGTCACC |
Stx1 cp | stx1 capture probe | 406 → | X - (CTCT)GAAGGGCGGTTTAATAATCTACGGC |
Stx1 dp | stx1 detection probe | 433 → | ATTGTTGAACGAAATAATTTATATG(ACTG) - Y |
gene stx2 (AF500187) | |||
Stx2 Primer I | stx2 upper primer | 205 → | TTTCTTCGGTATCCTATTCC |
Stx2 Primer II | stx2 lower primer | 701 ← | CTGCTGTGACAGTGACAAAACGC |
Stx2 cp | stx2 capture probe | 392 → | X - (TCTT)GCTTGATGTCTATCAGGCGCGTTTT |
Stx2 dp | stx2 detection probe | 418 → | ACCATCTTCGTCTGATTATTGAGCA(TCGG) - Y |
gene eae (AF022236) | |||
Eae Primer I | eae upper primer | 25479 → | GGAACGGCAGAGGTTAATCTGCAG |
Eae Primer II | eae lower primer | 25805 ← | GGCGCTCATCATAGTCTTTC |
Eae cp | eae capture probe | 25568 → | X - (GCGA)GCTGGCATTTGGTCAGGTCGGAGCG |
Eae dp | eae detection probe | 25594 → | GTTACATTGACTCCCGCTTTACGGC(TTTA) - Y |
NC cp | Negative Control capture probe (acoC) | 192 → | X - ACTATCGACACGGCCCGCCTTGGAGAAGA |