Figure 5

EHEC DNA chip array assessment. Four individual assays, A1, A2, A3 and A4, were serially conducted with four various products of simplex PCR, etpC, stx1, stx2 and eae, respectively, used as targets, and with corresponding detection probes (10 nM). 0.4 nM of each purified PCR amplicon was sequentially applied onto the chip array functionalized with capture probes. Each column is an average of three independent determinations. NC is non-biotinylated negative control capture probe of acoC of B. subtilis.