Figure 3
From: Sample processing for DNA chip array-based analysis of enterohemorrhagic Escherichia coli (EHEC)
Electrophoretic analyses of PCR amplicons: etpC (219 pb); stx1 (302 bp); stx2 (519 bp); and eae (346 bp). A. Genomic DNAs from E. coli strains: (1) EDL933, (2) CB571, (3) 86–24, (4) DH5α, (5) MG1655, and (6) W3110, were applied as the PCR templates, respectively. Reaction conditions: 1-minute primer annealing at 57°C, and 2-minute elongation step. B. Genomic DNAs from E. coli strains: (1) EDL933, (2) CB571, (3) 86–24, (4) DH5α, were applied as the PCR templates, respectively. Reaction conditions: 2-minute primer annealing at 56°C, and 3-minute elongation step. NC is a blank PCR assay. L stands for DNA Ladder.