Large-scale functional expression of WT and truncated human adenosine A2A receptor in Pichia pastoris bioreactor cultures

Table 1 Cell densities and specific binding for the A_2AR constructs expressed in flask and bioreactor cultures

Construct	Cell density (OD₆₀₀)*		Maximum specific binding (pmol/mg)
	Flask	Bioreactor	Flask	Bioreactor
WT A_2AR	14	75	32.5 ± 9.3	112.5 ± 2.7
A_2AR V334	14	80	82.9 ± 11.2	200.6 ± 17.6

ISSN: 1475-2859