Table 3 Comparative results of LLO production and purification by different investigators.
From: Nisin inducible production of listeriolysin O in Lactococcus lactis NZ9000
Source of LLO | Starter Culture volume (litre) | LLO yield (mg l-1) {Total yield (mg)} | Specific LLO haemolytic activity (HU per mg protein) pH 5.5 | Expression host | Plasmid expression system/inducer (If applicable) | Reference |
|---|---|---|---|---|---|---|
Culture supernatant | 27 | 0.022 {0.6} | 106 (pH 6) | Listeria monocytogenes a | N/A | [24] |
Culture supernatant | 3 | 1.6 {4.8} | 1.02 × 106 (pH 5.7) | Listeria innocua b | pERL3-503 (constitutive) | [26] |
Culture supernatant | 6 | 0.25 {1.5} | 2.6 × 105 | LLO-hypersecretor Listeria monocytogenesa | N/A | [25] |
Cell lysate | 1 | 4.5 {4.5} | 1.25 × 106 | Escherichia coli c | pET system/IPTG | [15] |
Cell lysate | 0.6 | 3.5 – 8 {2.1 – 4.8} | 1.8 × 106 | Escherichia coli d | pQE31 system/IPTG | [14] |
Cell lysate | 0.6 | 2.5 {1.5} | 2.16 × 106 | Escherichia coli e | pQE70 system/IPTG | [14] |
Cell lysate | 0.5 | -Single sonication treatment: 4.43 – 5.9 {2.215 – 2.95} -Double sonication approach: 9.3 – 12.9 {4.65 – 6.45} | 5 × 105 – 5 × 107 | Lactococcus lactis NZ9000d | NICE system (pNZPnisA:CYTO-LLO)/nisin | This study |