Figure 4
From: Nisin inducible production of listeriolysin O in Lactococcus lactis NZ9000
SDS-PAGE of purified LLO using Ni-NTA affinity chromatography. Lane 1 is the protein marker, lane 2 is culture lysate of L. lactis NZ9000 (pNZPnisA:CYTO-LLO) before passing through the column, lane 3 is the flow-through of the column, lanes 4 and 5 are two successive washes of the columns while lanes 6 and 7 are two successive elutions showing the purified His-tagged LLO.