Table 1 Primers used for PCR amplifications
From: Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris
ScR1fw | GTACGGCCCAGCCGGCCACGATGACCATAGATAACTACGAC |
|---|---|
ScR1bw | GTACGCGGCCGCTTATATTGCCAGCGTCGATG |
PpR1fw | GTACGGCCCAGCCGGCCACGATGTCCGCCGCTCACGATATTTC |
PpR1bw | GTACGCGGCCGCATCATTTCACCATTAC |
PpR1fwPro | GTACGCGGCCGCTGGTTTGCCGTTTCATCAGC |
PpR1bwPro | GTACGGATCCCGCAAGAGTGGGGCATAAAAT |
PpR2fw | GTACGGCCCAGCCGGCCACGATGAGTAAAAGACTTCCACAGAGAGATGC |
PpR2bw | GTACGCGGCCGCCTTTGGTTTTTCCGGCTCGTATTTG |
PpR2fwPro | GTACGGATCCCTGGTAATATCTTGTAACTAATCC |
PpR2bwPro | GTACGGATCCATCCCCAAGACTTCTACACTACAT |
PpR3fw | GTACGGCCCAGCCGGCCACGATGTCCGTGTTTACGCCAATAGAG |
PpR3bw | GTACGCGGCCGCTCTCTGTATTCCTATCCATCGTATC |
PpR3fwPro | GTACGGATCCGTGGCGTGTATGAGGTAAAACTGC |
PpR3bwPro | GTACGGATCCAGGGCTAATGACGGACAACTTCTT |
PpR4fw | GTACGGCCCAGCCGGCCACGATGCTCATATTACGTAATAC |
PpR4bw | GTACGCGGCCGCGAGGGCTCTTAACAAATAC |
PpR4fwPro | GTACGGATCCGCCTCGTCACCTTCGTCATCT |
PpR4bwPro | GTACGGATCCTTGCCTCACTGTGTCTTTCTTTAT |
PpR5fw | GTACGGCCCAGCCGGCCACGATGTTTACGGGAATAGTGGAAAT |
PpR5bw | GTACGCGGCCGCCCCTAGTTAGATATAAAATGGAG |
PpR5fwPro | GTACGGATCCCCCATTGCGAGCGACCTTC |
PpR5bwPro | GTACGGATCCAAATTTTTCCATCACTTATC |
PpR7fw | GTACGGCCCAGCCGGCCACGATGTCATTTGTGCCCTTTCTTG |
PpR7bw | GTACGCGGCCGCGTGGTCGGTATGCCAGTTATAG |
PpR7fwPro | GTACGGATCCCGATCTGGGTTATTCTCTTCTTTA |
PpR7bwPro | GTACGGATCCTCTTTGGACGGCGGTGTG |