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Table 1 Bacterial strains and plasmids.

From: Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by Lactococcus lactis

Strains

Genotype

 

Reference

E. coli DH5α

Wild type, plasmid free

 

L. lactis MG1363

Wild type, plasmid free

Gasson, 1983

L. lactis NZ9000

MG1363 (nisRK genes into chromosome), plasmid free

Kuipers et al., 1998

NZ(pSEC:huIP-10)

MG1363 (nisRK genes into chromosome), pSEC:huIP-10

This work

Plasmids

Replicon

Plasmid characteristics

Reference

pCR:TOPO

ori pUC

Apr

Invitrogen

pCR:huIP-10

ori pUC

Apr, DNA fragment encoding the IP-10 mature moiety

This work

pSEC:E7

pWV01

Cmr; gene expressed from PnisA encodes SPUsp-E7 precursor

Bermúdez-Humarán et al., 2002

pSEC:huIP-10

pWV01

Cmr; gene expressed from PnisA encodes SPUsp-huIP-10 precursor

This work