From: Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by Lactococcus lactis
Strains | Genotype | Â | Reference |
---|---|---|---|
E. coli DH5α | Wild type, plasmid free |  | |
L. lactis MG1363 | Wild type, plasmid free | Gasson, 1983 | |
L. lactis NZ9000 | MG1363 (nisRK genes into chromosome), plasmid free | Kuipers et al., 1998 | |
NZ(pSEC:huIP-10) | MG1363 (nisRK genes into chromosome), pSEC:huIP-10 | This work | |
Plasmids | Replicon | Plasmid characteristics | Reference |
pCR:TOPO | ori pUC | Apr | Invitrogen |
pCR:huIP-10 | ori pUC | Apr, DNA fragment encoding the IP-10 mature moiety | This work |
pSEC:E7 | pWV01 | Cmr; gene expressed from PnisA encodes SPUsp-E7 precursor | Bermúdez-Humarán et al., 2002 |
pSEC:huIP-10 | pWV01 | Cmr; gene expressed from PnisA encodes SPUsp-huIP-10 precursor | This work |