Figure 2
From: Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by Lactococcus lactis
Expression analysis of IP-10 secretion by recombinant Lactococcus lactis. Protein extracts from induced and noninduced cultures of recombinant Lactococcus lactis NZpSEC:huIP-10 were prepared from cell-free samples and analyzed by Western blotting using anti-IP-10. Mature IP-10 was detected in all the induced (+) cultures in the 10 kDa range as expected. No signal was found for noninduced cultures (-) of recombinant L. lactis NZpSEC:huIP-10. No immature or incomplete forms of IP-10 were detected. M, protein molecular marker.