Table 2 XR, XDH, XK activities, ethanol and xylitol productivity of H. polymorpha transformants and control strain

XDH

XK

NADPH/K_M (μM)

NADH/K_M (μM)

Ethanol

Xylitol

XRn

0.091 ± 0.005/9 ± 0.5

0.016 ± 0.001/100 ± 5.5

0.551 ± 0.035

-

6.3 ± 0.3/2.75 ± 0,11

3.6 ± 0.2/1.56 ± 0.1

XRm

0.01 ± 0.001/152 ± 8.4

0.014 ± 0.001/112 ± 6.5

0.504 ± 0.028

-

9.8 ± 0.4/4.45 ± 0.19

3.2 ± 0.2/1.45 ± 0.07

XRn/XDH

-

-

1.3 ± 0.07

-

12 ± 0.5/5.7 ± 0.3

3.1 ± 0.2/1.48 ± 0.07

XRm/XDH

-

-

1.485 ± 0.087

-

18.4 ± 0.9/8.76 ± 0.35

1.6 ± 0.1/0.76 ± 0.04

XRn/XDH/XK

-

-

-

0.375 ± 0.021

13.8 ± 0.6/6.4 ± 0.3

2.8 ± 0.1/1.3 ± 0.06

XRm/XDH/XK

-

-

-

0.366 ± 0.019

54.7 ± 2.7/21.9 ± 1.1

0.9 ± 0.04/0.36 ± 0.02

CBS4732

0.034 ± 0.025/7 ± 0.4

0.012 ± 0.001/85 ± 4.7

0.697 ± 0.049

0.156 ± 0.009

7.5 ± 0.4/3.3 ± 0.2

4.2 ± 0.2/1.8 ± 0.09