Skip to main content

Table 1 Strains and plasmids used in the present investigation.

From: Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha

Strains Genotype Reference
CBS4732 leu 2-2 [27]
Δxyl1 Δxyl1::LEU2Sc [23]
XRn Δxyl1, pX1N-Z (GAPp-XYL1-AOXt) This study
XRm Δxyl1, pX1M-Z (GAPp-XYL1mod-AOXt) This study
XRn/XDH Δxyl1, pX1N-Z-X2 (GAPp-XYL1-AOXt, GAPp-XYL2-XYL2t) This study
XRm/XDH Δxyl1, pX1M-Z-X2 (GAPp-XYL1mod-AOXt, GAPp-XYL2-XYL2t) This study
XRn/XDH/XK Δxyl1, pX1N-Z-X2 (GAPp-XYL1-AOXt, GAPp-XYL2-XYL2t), pGLG61/HpXYL3 (GAPp-XYL3-AOXt) This study
XRm/XDH/XK Δxyl1, pX1M-Z-X2 (GAPp-XYL1mod-AOXt, GAPp-XYL2-XYL2t), pGLG61/HpXYL3 (GAPp-XYL3-AOXt) This study