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Table 1 Strains and plasmids used in the present investigation.

From: Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha

Strains

Genotype

Reference

CBS4732

leu 2-2

[27]

Δxyl1

Δxyl1::LEU2Sc

[23]

XRn

Δxyl1, pX1N-Z (GAPp-XYL1-AOXt)

This study

XRm

Δxyl1, pX1M-Z (GAPp-XYL1mod-AOXt)

This study

XRn/XDH

Δxyl1, pX1N-Z-X2 (GAPp-XYL1-AOXt, GAPp-XYL2-XYL2t)

This study

XRm/XDH

Δxyl1, pX1M-Z-X2 (GAPp-XYL1mod-AOXt, GAPp-XYL2-XYL2t)

This study

XRn/XDH/XK

Δxyl1, pX1N-Z-X2 (GAPp-XYL1-AOXt, GAPp-XYL2-XYL2t), pGLG61/HpXYL3 (GAPp-XYL3-AOXt)

This study

XRm/XDH/XK

Δxyl1, pX1M-Z-X2 (GAPp-XYL1mod-AOXt, GAPp-XYL2-XYL2t), pGLG61/HpXYL3 (GAPp-XYL3-AOXt)

This study