Minimal information: an urgent need to assess the functional reliability of recombinant proteins used in biological experiments

Table 2 Experimental information check list. MI for each experiment is in bold, optional information is in italics.

Experiment type	Analyses to perform for evaluating proteins before starting the experiments	Protocol references (from a list of accepted standard protocols for each experiment type that guarantee output congruency)	Equipments (define minimal requisites of the devices in terms of quality, maintenance, controls)
Protein pull-down	SDS PAGE; AI; SEC; DLS; CD far UV; CD near UV; FT-IR; NMR;	SDS: protocol 1 (or protocol 2,..) AI protocol 1	SDS: Hoefer minigel.... AI: Spectrofluorimeter Jasco ..... SEC: column xy and FPLC model z.....
Monoclonal Ab purification	SDS PAGE; AI; SEC; DLS; CD far UV; CD near UV;
Protein purification	SDS PAGE; AI; SEC; DLS; CD far UV; CD near UV; FT-IR; NMR;
SPR
ITC
ELISA
Enzymatic assay
Protein refolding
Immunoprecipitation
Immunofluorescence
Immunohistochemistry
Proximity ligation

The corresponding pictures (SDS-gels, chromatographic profiles, fluorimeter spectra,.....) would be embedded in the metadata form.
Abbreviations: Aggregation Index (AI) (16), Size Exclusion Chromatography (SEC), Dynamic Light Scattering (DLS), Circular Dichroism (CD), Fourier-Transform InfraRed (FT-IR), Nuclear Magnetic Resonance (NMR).

ISSN: 1475-2859