Skip to main content
Figure 1 | Microbial Cell Factories

Figure 1

From: Development of an improved Pseudoalteromonas haloplanktis TAC125 strain for recombinant protein secretion at low temperature

Figure 1

In gel analysis of extra-cellular proteolytic activities from culture supernatants of P. haloplanktis TAC125 wild type and gspE mutant strain. Panel A: Zymography of P. haloplanktis TAC125 wild type culture supernatants collected at early (24 h) (lane 1), and middle (32 h) (lane 2) exponential phase. In this experiment the zymographic developing time was 18 h, a condition that assures the detection of all proteases contained in the sample. Panel B: Protease zymography of a P. haloplanktis TAC125 wild type culture supernatant, collected at 24 h, untreated (NONE) and treated with protease inhibitors (10 mM EDTA, 10 mM PMSF, and the combination of the two inhibitors both at 10 mM final concentration). In this experiment a zymographic developing time of 12 h was chosen, this condition allows a clearer visualization and comparison of the proteases contained in the different samples. Panel C: Protease zymography of P. haloplanktis TAC125 gspE mutant culture supernatants collected at early (24 h) (lane 1), and middle (32 h) (lane 2) exponential phase, the zymographic developing time was 18 h. Panel D: Protease zymography of a P. haloplanktis TAC125 gspE mutant culture supernatant, collected at 24 h, untreated (NONE) and treated with protease inhibitors, the zymographic developing time was 12 h).

Back to article page