Figure 3

Relative distribution of absolute fluxes of A) B. subtilis wild type at dilution rates of 0.05 h^-1 (top), 0.2 h^-1 (middle) and 0.4 h^-1 (bottom) and B) B. licheniformis T218a (top), B. licheniformis T380B (middle) and B. subtilis natto (bottom) at dilution rate 0.2 h^-1. Fluxes were determined by ¹³C-constrained flux analysis from two separate experiments with 100% [1-¹³C]glucose and with a mixture of 20% [U-¹³C] and 80% unlabeled glucose (Additional file 2) along with the physiological data of the chemostat experiments (additional files 5 and 7). Generally, the 95% confidence intervals were between 10 and 15% of the values shown for the major fluxes. Larger confidence intervals were estimated for reactions with low fluxes. To facilitate cross species comparison, fluxes are normalized to the glucose consumption rates given in (additional files 5 and 7). Arrowheads indicate flux directions.