Figure 1

Relative distribution of absolute fluxes in glucose batch cultures of A) B. subtilis wild type (top), spo0A (middle-top), sigE (middle-bottom) mutants and natto (bottom) and B) B. licheniformis T218a (top), B. licheniformis T380B (middle-top), B. amyloliquefaciens (middle-bottom) and B. pumilus (bottom). Fluxes were determined by ¹³C-constrained flux analysis from two separate experiments with 100% [1-¹³C]glucose and with a mixture of 20% [U-¹³C] and 80% unlabeled glucose (Additional file 2) along with the physiological data given in Table 2. Generally, the 95% confidence intervals were between 10 and 15% of the values shown for the major fluxes. Larger confidence intervals were estimated for reactions with low fluxes. To facilitate cross species comparison, fluxes are normalized to the glucose consumption rates given in Table 2. Arrowheads indicate flux direction.