Regulation of xylose metabolism in recombinant Saccharomyces cerevisiae

Salusjärvi, Laura; Kankainen, Matti; Soliymani, Rabah; Pitkänen, Juha-Pekka; Penttilä, Merja; Ruohonen, Laura

doi:10.1186/1475-2859-7-18

Microbial Cell Factories

Table 2 Proteins which were differentially translated in the glucose repressed, glucose derepressed and xylose-grown cells, identified from the 2-DE gels [Additional files 14 and 15].

From: Regulation of xylose metabolism in recombinant Saccharomyces cerevisiae

				log2 X72/G24		log2 X72/G5		log2 G24/G5
Swiss Prot Id	Protein^a	ORF	Function	2D	array	2D	array	2D	array
P32316	Ach1p	YBL015W	acetyl-CoA hydrolase	0.0	-1.0	3.4	2.7	3.4	3.7
P00330	Adh1p_a	YOL086C	alcohol dehydrogenase	1.4	0.9	0.2	0.3	-1.2	-0.6
P00330	Adh1p_b	YOL086C	alcohol dehydrogenase	2.1	0.9	0.5	0.3	-1.6	-0.6
P00331	Adh2p	YMR303C	alcohol dehydrogenase	4.8	-2.6	5.7	1.4	0.9	4.0
P47143	Ado1p_a	YJR105W	adenosine kinase	-1.1	0.4	-1.4	-1.3	-0.2	-1.7
P47143	Ado1p_b	YJR105W	adenosine kinase	-1.4	0.4	-1.9	-1.3	-0.5	-1.7
P38013	Ahp1p	YLR109W	thioredoxin peroxidase	-0.4	0.0	0.5	-0.2	1.0	-0.2
P54114	Ald3p	YMR169C	aldehyde dehydrogenase	0.4	0.8	1.8	1.8	1.4	1.0
P46367	Ald4p	YOR374W	aldehyde dehydrogenase (NAD⁺)	1.7	-0.2	3.6	1.0	1.9	1.1
P54115	Ald6p_a	YPL061W	aldehyde dehydrogenase (NADP⁺)	-0.9	-2.6	0.2	-1.9	1.0	0.7
P54115	Ald6p_b	YPL061W	aldehyde dehydrogenase (NADP⁺)	-2.0	-2.6	-2.8	-1.9	-0.8	0.7
P28777	Aro2p	YGL148W	chorismate synthase	-0.7	0.4	-1.4	-1.4	-0.7	-1.8
P53090	Aro8p	YGL202W	aromatic-amino-acid transaminase	-1.7	1.5	-0.6	-1.0	1.1	-2.5
P38011	Asc1p	YMR116C	molecular function unknown	-0.6	1.8	-2.4	-1.1	-1.8	-3.0
P07251	Atp1p	YBL099W	hydrogen-transporting ATP synthase	0.1	-0.2	1.8	0.7	1.7	0.9
P00830	Atp2p	YJR121W	hydrogen-transporting ATP synthase	0.2	-0.4	1.6	0.9	1.4	1.2
P38891	Bat1p	YHR208W	branched-chain-amino-acid transaminase	1.9	3.9	0.4	0.7	-1.5	-3.2
P07256	Cor1p_a	YBL045C	ubiquinol-cytochrome-c reductase	0.6	-0.2	1.3	1.0	0.8	1.2
P07256	Cor1p_b	YBL045C	ubiquinol-cytochrome-c reductase	0.3	-0.2	1.3	1.0	1.0	1.2
P07256	Cor1p_c	YBL045C	ubiquinol-cytochrome-c reductase	-0.1	-0.2	1.3	1.0	1.5	1.2
P06115	Ctt1p	YGR088W	catalase	1.4	1.8	3.4	2.4	2.0	0.6
P31373	Cys3p	YAL012W	cystathionine gamma-lyase	-2.3	-0.6	-2.9	-3.1	-0.6	-2.4
P32582	Cys4p_a	YGR155W	cystathionine beta-synthase	-2.5	-0.8	-0.2	-1.1	2.3	-0.3
P32582	Cys4p_b	YGR155W	cystathionine beta-synthase	-0.9	-0.8	-1.7	-1.1	-0.9	-0.3
P06634	Ded1p	YOR204W	RNA helicase	0.5	0.3	-2.2	0.5	-2.7	0.2
P14306	Dka1p	YLR178C	carboxypeptidase Y inhibitor	-1.2	-0.6	1.7	0.8	2.9	1.4
P39976	Dld3p	YEL071W	D-lactate dehydrogenase (cytochrome)	-2.0	-0.2	-2.4	-0.6	-0.3	-0.4
P00924	Eno1p_a	YGR254W	phosphopyruvate hydratase	1.5	0.6	5.2	0.5	3.7	-0.1
P00924	Eno1p_b	YGR254W	phosphopyruvate hydratase	3.5	0.6	5.7	0.5	2.2	-0.1
P00924	Eno1p_c	YGR254W	phosphopyruvate hydratase	1.2	0.6	2.6	0.5	1.3	-0.1
P14065	Gcy1p	YOR120W	aldo-keto reductase	2.0	0.3	3.7	3.1	1.7	2.8
Q00055	Gpd1p	YDL022W	glycerol-3-phosphate dehydrogenase	0.7	0.0	1.9	0.2	1.2	0.2
P00950	Gpm1p	YKL152C	phosphoglycerate mutase	1.7	0.5	1.6	-0.1	0.0	-0.7
P38625	Gua1p	YMR217W	GMP synthase	-0.8	1.3	-1.8	-1.0	-1.0	-2.3
P15454	Guk1p	YDR454C	guanylate kinase	-1.7	0.3	-1.4	-1.3	0.3	-1.6
P15992	Hsp26p_a	YBR072W	heat shock protein	0.1	-0.2	3.0	2.0	2.9	2.2
P15992	Hsp26p_b	YBR072W	heat shock protein	-0.6	-0.2	2.2	2.0	2.8	2.2
P04807	Hxk2p_a	YGL253W	hexokinase	2.6	2.9	10.7	-0.5	8.1	-3.4
P04807	Hxk2p_b	YGL253W	hexokinase	0.7	2.9	-1.1	-0.5	-1.8	-3.4
P28241	Idh2p	YOR136W	isocitrate dehydrogenase (NAD+)	-0.9	-0.5	0.7	0.2	1.7	0.7
P00817	Ipp1p	YBR011C	inorganic diphosphatase	-1.7	-0.5	-1.5	-0.9	0.3	-0.5
P53598	Lsc1p	YOR142W	succinate-CoA ligase (ADP-forming)	-0.5	-0.4	0.6	0.1	1.1	0.4
P36060	Mcr1p	YKL150W	cytochrome-b5 reductase	1.8	0.1	3.3	1.5	1.4	1.4
P05694	Met6p	YER091C	methionine synthase	-2.5	-1.3	-2.9	-1.0	-0.4	0.3
P04147	Pab1p	YER165W	poly(A) binding protein	0.4	0.2	-1.1	-0.3	-1.6	-0.5
P06169	Pdc1p_a ^b	YLR044C	pyruvate decarboxylase	-1.7	2.0	-1.8	-0.2	0.0	-2.2
P06169	Pdc1p_b ^b	YLR044C	pyruvate decarboxylase	-2.1	2.0	-1.2	-0.2	0.9	-2.2
P17967	Pdi1p	YCL043C	protein disulfide isomerase	-0.7	-0.1	-2.6	-0.5	-1.9	-0.4
P00560	Pgk1p_a	YCR012W	phosphoglycerate kinase	1.1	0.6	1.8	0.1	0.7	-0.5
P00560	Pgk1p_b	YCR012W	phosphoglycerate kinase	1.9	0.6	2.6	0.1	0.7	-0.5
P34227	Prx1p	YBL064C	thioredoxin peroxidase	-0.2	-0.9	1.7	0.7	1.9	1.6
Q12335	Pst2p	YDR032C	molecular function unknown	-0.6	-0.1	0.8	-0.4	1.5	-0.3
P07703	Rpc5p	YPR110C	DNA-directed RNA polymerase	-0.3	0.5	-1.5	-1.3	-1.2	-1.8
P26783	Rps5p	YJR123W	structural constituent of ribosome	-0.6	0.7	-3.0	-0.6	-2.4	-1.2
P19358	Sam2p	YDR502C	methionine adenosyltransferase	-2.2	-2.0	-2.5	-2.6	-0.3	-0.6
P07283	Sec53p_a	YFL045C	phosphomannomutase	-0.1	0.2	-2.5	-1.6	-2.4	-1.8
P07283	Sec53p_b	YFL045C	phosphomannomutase	-1.1	0.2	-2.0	-1.6	-0.8	-1.8
P33330	Ser1p	YOR184W	phosphoserine transaminase	-0.8	-0.3	-0.9	-1.0	-0.1	-0.8
P37291	Shm2p	YLR058C	glycine hydroxymethyltransferase	0.1	-0.1	-1.5	0.6	-1.6	0.7
Q03144	Sno1p	YMR095C	molecular function unknown	0.3	0.4	-0.8	2.0	-1.1	1.6
P00447	Sod2p	YHR008C	manganese superoxide dismutase	-0.5	-0.2	1.9	1.1	2.4	1.2
P15705	Sti1p	YOR027W	Hsp90 cochaperone	0.6	0.2	1.7	0.3	1.1	0.1
P23254	Tkl1p	YPR074C	transketolase	-0.6	1.1	-1.0	-0.6	-0.5	-1.7
P00942	Tpi1p	YDR050C	triose-phosphate isomerase	-1.1	0.2	0.5	-0.1	1.5	-0.3
P17649	Uga1p	YGR019W	4-aminobutyrate transaminase	-0.8	-0.1	1.4	0.2	2.2	0.3
Q12363	Wtm1p_a	YOR230W	transcriptional modulator	0.1	-0.1	1.7	0.2	1.6	0.3
Q12363	Wtm1p_b	YOR230W	transcriptional modulator	-0.1	-0.1	1.3	0.2	1.4	0.3
P23180	Yhl021p	YHL021C	molecular function unknown	-0.5	-0.8	1.6	0.6	2.1	1.3
P35691	Ykl056cp	YKL056C	molecular function unknown	-1.0	0.5	-1.8	-1.4	-0.9	-1.9
Q04869	Ymr315wp	YMR315W	molecular function unknown	2.5	2.3	3.1	1.8	0.6	-0.4

Log2 values of the protein abundance ratios, and the expression ratios for the corresponding gene of cells grown on xylose for 72 h (X72) to cells grown on glucose for 24 h (G24), or 5 h (G5), and of cells grown on glucose for 24 h to cells grown on glucose for 5 h are given. Proteins whose encoding genes showed a statistically significant expression difference in the samples studied are marked with bold text.
^aDifferent pI forms of a protein indicated by a, b and c
^bThese spots of Pdc1p had substantially lower molecular weight compared with the major Pdc1p protein spot identified earlier [15] and are likely either degraded or post-translationally processed forms of the enzyme.

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ISSN: 1475-2859

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