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Table 1 Alkaloids produced from hyoscyamine with the tagged and untagged H6H after 2 and 15 h of incubation.

From: Expression of Brugmansia candida Hyoscyamine 6beta-Hydroxylase gene in Saccharomyces cerevisiae and its potential use as biocatalyst

 

6β-hydroxyhyoscyamine (%)

Scopolamine (%)

Time (h)

2

15

2

15

Untagged H6H

53.7

83.3

-

7,6

Tagged H6H

15.0

35.7

-

-

Negative control

-

-

-

-

  1. The percentage values of 6β-hydroxyhyoscyamine and scopolamine are the means of three independent determinations which differs in no more than 10%