A genomic search approach to identify esterases in Propionibacterium freudenreichii involved in the formation of flavour in Emmental cheese

Table 1 Strains, broths, plasmids, and primers used in this study.

Broths or strains or plasmids or primers	Relevant features	References or remarks
P. freudenreichii
CIP103027^T	P. freudenreichii sequenced type strain	Institut Pasteur^£
E. coli
DH10_B	Host for pDONR221	Novagen^#
B = BL21 (DE3) pLysS	Host for expression vectors	Novagen^#
R = Rosetta (DE3)	Host for expression vectors	Novagen^#
O = OrigamiB (DE3) pLysS	Host for expression vectors	Novagen^#
C = C41 (DE3) pRos	Host for expression vectors	Avidis SA^$
Broths
LB	Miller's LB Broth Base	Invitrogen
SB	Superior Broth	Euromedex^§
TB	Terrific Broth	Invitrogen
YEL	Yeast extract lactate broth	Malik et al [21]
Plasmids
pDONR221	Gateway entry vector	Invitrogen
pETG20A	Expression vector	A. Geerlof*
pETG41A	Expression vector	A. Geerlof*
Primers^a
Forward	GGGGACAAGTTTGTACAAAAAAGCAGGCTCGGAAAACCTGTACTTCCAGGGTxx	This study
Reverse (attB2)	xxGGGGACCACTTTGTACAAGAAAGCTGGGTC	Invitrogen

^£Institut Pasteur, Paris, France. ^#Novagen, Gibbstown, NJ, USA. ^$Avidis SA, Saint-Beauzire, France. ^§Euromedex, Mundolsheim, France. *A. Geerlof, EMBL, Heidelberg, Germany.^axx indicates the start and the end of each P. freudenreichii sequence cloned.

ISSN: 1475-2859