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Table 1 E. coli strains used and their genotypes

From: Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red®/ET® Recombination

E. colistrain

Genotype

Reference

MG1655

wild-type; F- lambda- ilvG rfb50 rph1

[31]

MC4100

F- araD139 Δ(argF-lac) U169 rpsL150 relA1 flb-5301 fruA25 deoC1 ptsF25

[7]

TK2204

F- thi rha lacZ(am) nagA kdpA4 trkA405 trkD1

[10]

MG1655 rpsL150

F- lambda- ilvG rfb50 rph1 rpsL150 (StrR)

this work

MG1655 rpsL150 kdpA4:rpsL-neo

F- lambda- ilvG rfb50 rph1 rpsL150 kdpA::rpsL-neo (KanR, StrS)

this work

MG1655 rpsL150 kdpA4

F- lambda- ilvG rfb50 rph1 rpsL150 (StrR) kdpA4

this work