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Figure 2 | Microbial Cell Factories

Figure 2

From: Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red®/ET® Recombination

Figure 2

Primer design to introduce the replacement G1033 to A in kdpA encoding KdpA/G345S. The homologous parts of the homology arms to kdpA are indicated as well as the primers for introduction and verification of the rpsL-neo cassette inserted into the kdpA gene. In the lower part, the composition and the sizes of the linear DNA-fragments for recombination are indicated. The asterisk represents the mutated base in kdpA. In the upper part, the sizes of DNA-fragments confirming the introduction or loss of the rpsL-neo cassette are indicated. nt = nucleotides.

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Microbial Cell Factories

ISSN: 1475-2859

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