Coutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system

Table 5 Escherichia coli strains and plasmids used in this work.

Strain or plasmid	Relevant genotype	Reference
JM101	supE, thi Δ(lac-proAB), F' tra D36 proA⁺ proB⁺ lac I^q lac ZΔM15	[31]
PB12	This strain was derived from PB11PTS^-Glc^-, a ptsHIcrr deletion derivative of strain JM101. PB12 has the same genotype of PB11 and at least three additional mutations (arcB, rpoS and a mutation responsible for the upregulation of genes involved in the ppGpp metabolism) that appeared during the selection of this fast growing mutant on glucose.	[7,9,21]
PB12aroB^-	PB12aroB::cat	This work
pRW300	aroG^fbr is under the control of the IPTG inducible promoter lacUV5; carrying tetracycline resistance. Replication origin of pBR322.	[16] [10]
pCLtktA	tktA is under its constitutive promoter carrying spectinomycin resistance. Replication origin of pACYC184.	[16]

ISSN: 1475-2859