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Table 2 Kinetic and stoichiometric parameters for strain JM101 and its derivative PB12 PTS-Glc+, using a mixture of two carbon sources.

From: Coutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system

 

JM101

PB12

Carbon sources

μ hr-1

Yx/s g/mmolC

qs mmolC/gDCW hr

acetmax g/L

μ hr-1

Yx/s g/mmolC

qs mmolC/gDCWhr

acetmax g/L

Glucose + Arabinose

0.72 (0.64)

0.0180 (0.0.012)

40.68 (7.64)

0.30

0.55

0.0144

38.19

0.30

Glucose + Glycerol

0.72 (0.45)

0.0171 (0.006)

43.11 (8.55)

0.13

0.50 (0.20)

0.0136 (0.017)

38.23 (9.24)

0.03

Glucose + Gluconate

0.72 (0.33)

0.00108 (0.0133)

70.33 (20.62)

0.49

0.51

0.0125

40.80

0.39

  1. Different parameters (μ, qs and Yx/s, see Methods) were determined for strain JM101 and its derivative PB12 PTS-Glc+ using mixture of two carbon sources. The numbers in parentheses indicate data for the specific strain in those stages in which only the remaining carbon source was available. In the case of JM101, the remaining carbon source was arabinose, glycerol, or gluconate, whereas glycerol was for PB12.
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Microbial Cell Factories

ISSN: 1475-2859

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