Figure 1

(A) Schematic highlighting the major steps in a cDNA microarray experiment [8]. Briefly, RNA from biological samples is isolated and checked for purity. Of the two RNA samples shown, one is considered the 'test' and the other the 'control'. Each sample is labeled with a different fluorescent dye, indicated by the green and red colors. The two samples are then mixed and hybridized onto a spotted microarray slide. Once the slide is washed, it is scanned at two different wavelengths, each corresponding to one of the dyes. (B) Image of scanned microarray wherein a number of distinct colors can be seen. Each color is representative of a certain amount of binding. For the image shown, the test sample was labeled with Cy5 and the control sample was labeled with Cy3.